biotinylated universal antibody (Vector Laboratories)

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Vector Laboratories biotinylated universal antibody
Biotinylated Universal Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7706 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/BA-1400-2%2E1/Biotinylated+Universal+Antibody+(Horse+Anti-Mouse%2FRabbit+IgG)/pm42883015-119-10-13
Average 96 stars, based on 7706 article reviews

biotinylated universal antibody - by Bioz Stars, 2026-11

96/100 stars

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Saline:

Article Title: Functional, histological, and molecular adaptations of forelimb muscles in a rat model of monocrotaline-induced heart failure.
Article Snippet: Histological changes in skeletal muscle after heart failure have been widely investigated in the lower limbs, including slow-to-fast fiber type transition, fast fiber-predominant atrophy, reduced capillary number, and increased collagen content.. However, histological changes in the upper limb muscles remain largely unexplored.. Given the prognostic significance of grip strength and upper limb muscle mass in heart failure, elucidating these changes is essential.

Incubation:

Article Title: The cannabidiol (CBD): Tetrahydrocanabinol (THC) concentration ratio is critical for neuroprotection and recovery following traumatic brain injury.
Article Snippet: .. incubation with a selective monoclonal neuronal NeuN antibody (dilution 1:1000 in 0.5% BSA) (Millipore Inc) or with a monoclonal astrocytic marker, glial fibrillary acidic protein (GFAP) overnight at 4o C. A biotinylated mouse antibody was used as a secondary antibody (Vector Laboratories). .. Sections were rinsed in PBS (three times) and placed in avidin-biotinylated enzyme complex (ABC) solution (Vector Laboratories, Burlingame, CA) for 1 hr.

Article Title: Testicular Expression of Vitamin-D Receptor and Vitamin-D-Metabolizing Enzymes (CYP2R1, CYP27A1, and CYP27B1) in Normal and Cryptorchid Horses.
Article Snippet: .. After a washing step with PBS for 15 min, the biotinylated universal antibody (VectorLaboratories, Burlingame, CA, USA) was added for 30 min at RT, followed, after a new 15-min PBS wash, by 30 min of incubation with Vectastain Elite ABC Reagent (VectorLaboratories, Burlingame, CA, USA). .. After washing in PBS, staining was visualized by incubating the sections in DAB solution (Vector-Laboratories, Burlingame, CA, USA).

Article Title: Intricate mesoscale organization of the anterior commissure in the rhesus monkey, with implications for neuroimaging.
Article Snippet: .. In brief, this involved incubation overnight at room temperature in primary antibody (1:500, mouse antibody; ImmunoStar, Hudson, WI), followed by 1 h incubation at room temperature in 0.44% biotinylated anti-mouse horse-made IgG (Vector Laboratories, Burlingame, CA), and re-incubation (1 h at room temperature) in 0.8% peroxidase-labeled avidin biotin complex (ABC Standard kit, Vector). .. The bound peroxidase was revealed by incubating the section for 3 min at room temperature, in 0.05% solution of DAB (Sigma, St. Louis, MO) to which 0.005% hydrogen peroxide was added.

Marker:

Labeling:

Article Title: N-terminally acetylated Met11-Tau: a new pathological truncated Tau species with functional relevance in Alzheimer's disease
Article Snippet: Brain sections were washed with PBS-Triton (0.2%), treated for 30 min with 0.3% H 2 O 2 and nonspecific binding was blocked with MOM (Mouse on Mouse) blocking reagent (Vector Laboratories, Burlingame, CA) or horse serum (1:100 in PBS; Vector Laboratories) for 1 h. The sections were then incubated with the primary antibody (Table S2) in PBS-Triton 0.2% overnight at 4°C. .. After 3 × 10 min washes, labeling was amplified using a biotinylated anti-mouse or anti-rabbit IgG (1:400 in PBS-Triton 0.2%; Vector Laboratories) for 1 h, followed by the ABC kit (1:400 in PBS; Vector Laboratories), and labeling was completed using 0.5 mg/mL DAB (Vector Laboratories) in 50 mmol/l Tris–HCl, pH 7.6, containing 0.075% H 2 O 2 . .. Brain sections were mounted on SuperFrost slides, dehydrated through a graded series of alcohol and toluene, and then mounted with Vectamount medium (#H5000; Vector Laboratories) and coverslips (#LCO2460M; Labelians) for microscopic analysis.

Amplification:

Avidin-Biotin Assay:

Article Title: Lipopolysaccharide uptake is augmented in lipopolysaccharide-tolerant mouse macrophage-like cells via increased CD14 expression.
Article Snippet: .. The biotinylated antibody was detected using an avidin-biotin system with the VECTASTAIN Elite ABC Kit (Vector Laboratories, Newark, CA, USA) and TMB Peroxidase Substrate Kit (Vector Laboratories). ..

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